Primary invagination can also be disrupted by treating embryos with a monoclonal antibody (MAb 183) that recognizes the protein hyalin, the predominant protein in the hyaline layer. The hyaline layer peels away from the epithelium in antibody treated embryos, and although mesenchyme cells can migrate in such embryos, invagination is completely blocked.
Click on the picture below to see a movie of an embryos treated with MAb 183.
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