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Images in the banner (from left to right and top to bottom): Adult C. elegans hermaphrodite [A. Cox]; DLG-1/Discs large::GFP during dorsal intercalation [M. Köppen]; Pdlg-1::GFP during ventral enclosure [M. Sheffield]; AJM-1 (green) and muscle (red) during elongation [P. Heid]; MOM-5/Dsh::GFP in the early embryo [T. Walston]; AJM-1 (green) and muscle (red) in an elongated embryo [A. Cox-Paulson]; HMP-1/alpha-catenin (green), AJM-1::GFP (red), DNA (blue) in a comma stage embryo [J. Simske]; VAB-10::ABD::GFP (actin) at 2-fold [R. Zaidel-Bar]; phalloidin staining in elongated embryo [M. Costa]
Welcome to the Hardin Lab! We use the C. elegans embryo as a model for investigating cell movement and cell adhesion during embryonic development. Understanding how cells move, and how they make and break adhesions has important implications for understanding birth defects during human development and for understanding cancer progression. To find out more, click here...

Our work is supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, National Institute of General Medical Sciences, NIH, and the Division of Integrative Organismal Biology, NSF.

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Postdoctoral position available. Click here for more information...

Recent News

January 2012
Cell migration during ventral enclosure
Ikegami, R., Simokat, K., Zheng, H., Dixon, L., Garriga, G., Hardin, J. and Culotti, J. (2012). Semaphorin and Eph receptor signaling guide a series of cell movements for ventral enclosure in C. elegans. Curr. Biol. 22:1–11. PubMed pdf

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November 2011
α-catenin review
Maiden, S.L. and Hardin, J. (2011). The secret life of α-catenin: moonlighting during morphogenesis. J. Cell Biol. 195:543–552. PubMed pdf

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January 2011
Loss of the RhoGAP SRGP-1 promotes the clearance of dead and injured cells
Neukomm, L.J., Frei, A.P., Cabello, J., Kinchen, J.M., Zaidel-Bar, R., Ma, Z., Haney, L.B., Hardin, J., Ravichandran, K.S., Moreno, S., and Hengartner, M.O. (2011). Loss of the RhoGAP SRGP1 promotes the clearance of dead and injured cells in Caenorhabditis elegans. Nature Cell Biol. 13:79-86. PubMed pdf

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ced-6 larvae accumulate cell corpses due to engulfment defects (arrowheads, left).
Engulfment defects are suppressed in ced-6;srgp-1 double mutants (right). [Lukas Neukomm]

Older News

November 2010
SRGP-1/srGAP regulates membrane protrusion during cell-cell adhesion
(Zaidel-Bar, R., Joyce, M.J., Lynch, A.M., Witte, K., Audhya, A., and Hardin, J. (2010). The F-BAR domain of SRGP-1 facilitates cell-cell adhesion during C. elegans morphogenesis. J. Cell Biol. 191, 761-9.) PubMed JCB In This Issue pdf f1000evaluation


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November 15, 2010 cover!
Embryo expressing SRGP-1::GFP (green) stained for actin (purple).
The inset shows extensive induced tubulations. [Ronen Zaidel-Bar].

August 2010
Intramolecular regulation of alpha-catenin's ability to bind actin
(Kwiatkowski, A.V., Maiden, S.L., Pokutta, S., Choi, H.-J., Benjamin, J.M., Lynch, A.M., Nelson, W.J., Weis, W.I., and Hardin, J. (2010). In vitro and in vivo reconstitution of the cadherin-catenin-actin complex from Caenorhabditis elegans. PNAS 107,14591-14596.)
PubMed pdf f1000evaluation
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maiden_phalloidin
August 17, 2010 cover!
Color adjusted image of a hmp-1(zu278) mutant
embryo stained with phalloidin. [Stephanie Maiden].

May 2010
Cadherins and L1CAMs during cell-cell adhesion and gastrulation
(Grana, T.M., Cox, E.A., Lynch, A.M., and Hardin, J. (2010). SAX-7/L1CAM and HMR-1/cadherin function redundantly in blastomere compaction and non-muscle myosin accumulation. Dev. Biol. 344, 731–744.)
PubMed pdf f1000evaluation


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Color adjusted image of a devitellinized hmr-1(RNAi);sax-7(eq1) C. elegans embryo. Blastomeres are loosely adherent and cell division orientations are abnormal. [Theresa Grana].


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