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C. elegans and Adhesion: Research or Reviews

Microscopy: Reviews and articles

Protocols: Protocols

Sea urchins: Research or Reviews

Older work: Articles

Teaching: Articles


Bold = Hardin lab member

Research articles - C. elegans
Maiden, S.L., Harrison, N., Keegan, J., Cain, B., Lynch, A.M., Pettitt, J., and Hardin, J. (2013). Specific conserved C-terminal amino acids of Caenorhabditis elegans HMP-1/α-catenin modulate F-actin binding independently of vinculin. J. Biol. Chem. 288:5694-706. PubMed: pdf

Lynch, A.M., Grana, T., Cox-Paulson, E., Couthier, A., Cameron, M., Chin-Sang, I., Pettitt, J., and Hardin, J. (2012). A genome-wide functional screen identifies MAGI-1 as an L1CAM-dependent stabilizer of apical junctions in C. elegans. Curr. Biol. 22, 1891–1899. PubMed pdf

Cox-Paulson, E., Walck-Shannon, E., Lynch, A.
, Yamashiro, S., Zaidel-Bar, R., Celeste C. Eno, C., Ono, S., and Hardin, J. (2012). Tropomodulin protects α-catenin-dependent junctional actin networks under stress during epithelial morphogenesis. Curr. Biol. 22:1500-1505. PubMed pdf

Ikegami, R., Simokat, K., Zheng, H., Dixon, L., Garriga, G., Hardin, J. and Culotti, J. (2012). Semaphorin and Eph receptor signaling guide a series of cell movements for ventral enclosure in C. elegans. Curr. Biol. 22:1–11. PubMed pdf

Neukomm, L.J., Frei, A.P., Cabello, J., Kinchen, J.M., Zaidel-Bar, R., Ma, Z., Haney, L.B., Hardin, J., Ravichandran, K.S., Moreno, S., and Hengartner, M.O. (2011). Loss of the RhoGAP SRGP1 promotes the clearance of dead and injured cells in Caenorhabditis elegans. Nature Cell Biol. 13:79-86. PubMed pdff1000evaluation

Zaidel-Bar, R., Joyce, M.J., Lynch, A.M
., Witte, K., Audhya, A., and Hardin, J. (2010). The F-BAR domain of SRGP-1 facilitates cell-cell adhesion during C. elegans morphogenesis. J. Cell Biol. 191, 761-9. PubMed JCB In This Issue pdf f1000evaluation

Kwiatkowski, A.V., Maiden, S.L., Pokutta, S., Choi, H.-J., Benjamin, J.M., Lynch, A.M., Nelson, W.J., Weis, W.I., and Hardin, J. (2010). In vitro and in vivo reconstitution of the cadherin-catenin-actin complex from Caenorhabditis elegans. PNAS 107:14591-14596. PubMed pdf f1000evaluation

Grana, T.M., Cox, E.A., Lynch, A.M., and Hardin, J.
(2010). SAX-7/L1CAM and HMR-1/cadherin function redundantly in blastomere compaction and non-muscle myosin accumulation. Dev. Biol. 344:731–744. PubMed pdf f1000evaluation

Giuliani, C., Troglio, F., T., Zucconi, A., Bai, Z., Patel, F.B., Zucconi, A., Malabarba, M.G., Disanza, A., Stradal, T., Cassata, G., Confalonieri, S., Hardin, J., Soto, M., Grant, B., and Scita, G. (2009). Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin dynamics and membrane trafficking during C. elegans oocyte growth and embryonic epidermal morphogenesis. PLoS Genetics 5(10): e1000675. PubMed pdf

King, R.S., Maiden, S.L., Hawkins, N.C., Kidd, A.R., Kimble, J., Hardin, J., and Walston, T.D.(2009). POP-1 asymmetry and morphogenesis defects in dsh-2 mutant embryos can be rescued by either the DIX or DEP domain of DSH-2. Dev. Biol. 328: 234-44. PubMed pdf

Hingwing, K., Lee, S., Nykilchuk, L., Walston, T., Hardin, J., and Hawkins, N. (2009). CWN-1 functions with DSH-2 to regulate C. elegans asymmetric neuroblast division in a β-catenin independent Wnt pathway. Dev Biol. 328: 245–56. PubMed pdf

Yamashiro, S., Cox, E.A., Ballie, D.L., Hardin, J. and Ono, S. (2008). Sarcomeric actin organization is synergistically promoted by tropomodulin, ADF/cofilin, AIP1, and profilin in C. elegans. J. Cell Sci. 121:3867-77. PubMed pdf

Ding, M., King, R.S., Berry, E.C., Wang, Y., Hardin, J., and Chisholm, A.D. (2008). The cell signaling adaptor protein EPS-8 is essential for C. elegans epidermal elongation and interacts with the ankyrin repeat protein VAB-19. PLoS ONE 3:e3346. PubMed pdf

Lockwood, C., Zaidel-Bar, R., and Hardin, J. (2008). The C. elegans Zonula Occludens ortholog ZOO-1 cooperates with the cadherin-catenin complex to recruit actin during epidermal morphogenesis. Curr. Biol. 18:1333-7. PubMed pdf f1000evaluation

Lockwood, C., Lynch, A., and Hardin, J. (2008). Dynamic analysis identifies novel roles for DLG-1 subdomains in AJM-1 recruitment and LET-413 dependent apical focusing. J. Cell Sci. 121: 1477-1487. PubMed pdf

Hardin, J., King, R., Thomas-Virnig, C., and Raich, W.B. (2008). Zygotic loss of ZEN-4/MKLP1 results in disruption of epidermal morphogenesis in the C. elegans embryo. Dev. Dyn. 237:830-6. PubMed pdf

Stevenson, T.O., Mercer, K., Cox, E.A., Szewczyk, N.J., Conley, C.A., Hardin, J.D., and Benian, G.M. (2007). unc-94 encodes a tropomodulin in C. elegans. J. Mol. Biol. 374, 936-50. PubMed pdf

Sheffield, M.S., Loveless, T., Hardin, J., and Pettitt, J. (2007). C. elegans Enabled exhibits novel interactions with N-WASP, Abl, and cell-cell junctions. Curr. Biol. 17, 1791-1796. PubMed pdf

Batchelder EL, Thomas-Virnig CL, Hardin JD, White JG. (2007). Cytokinesis is not controlled by calmodulin or myosin light chain kinase in the Caenorhabditis elegans early embryo. FEBS Letters 581:4337-41. PubMed pdf

Qadota H, Inoue M, Hikita T, Köppen M, Hardin JD, Amano M, Moerman DG, Kaibuchi K. (2007). Establishment of a tissue-specific RNAi system in C. elegans. Gene 400:166-73. PubMed pdf

Phillips, B. T., Kidd, A. R., 3rd, King, R., Hardin, J. and Kimble, J. (2007). Reciprocal asymmetry of SYS-1/beta-catenin and POP-1/TCF controls asymmetric divisions in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 104: 3231-6. PubMed pdf f1000evaluation

Lee, J.-Y., Marston, D.J., Halberstadt, A., Walston, T., Hardin, J. and B. Goldstein, B.  (2006). Wnt/Frizzled signaling regulates C. elegans gastrulation through activation of actomyosin contractility. Curr. Biol. 16,1986-97. PubMed pdf f1000evaluation

Walston, T., Guo, C., Proenca, R., Wu, M., Herman, M., Hardin, J., and Hedgecock, E. (2006). mig-5/Dsh controls cell fate determination and cell migration in C. elegans. Dev. Bio. 298, 485-497. PubMed pdf

Walston, T., Tuskey, C., Edgar, L., Hawkins, N., Ellis, G., Bowerman, B., Wood, W., and Hardin, J. (2004). Multiple Wnt signaling pathways converge to orient the mitotic spindle in early C. elegans embryos. Dev. Cell 7, 831-841. PubMed pdf

Thomas-Virnig, C.L., Simske, J.S., Sims, P.A., and Hardin, J. (2004). The inositol 1, 4, 5-trisphosphate receptor regulates epidermal cell migration in Caenorhabditis elegans. Curr. Biol. 14,1882-7. PubMed pdf

Pettitt, J., Cox, E.A., Broadbent, I.D., Flett, A., and Hardin, J. (2003). The Caenorhabditis elegans p120 catenin homologue, JAC-1, modulates cadherin-catenin function during epidermal morphogenesis. J Cell Biol 162, 15-22. PubMed pdf

Simske, J.S., Köppen, M., Sims, P., Hodgkin, J., Yonkof, A., and Hardin, J. (2003). The cell junction protein VAB-9 regulates adhesion and epidermal morphology in C. elegans. Nat Cell Biol 5, 619-625. PubMed pdf f1000evaluation

Köppen, M., Simske, J.S., Sims, P.A., Firestein, B.L., Hall, D.H., Radice, A.D., Rongo, C., and Hardin, J.D. (2001). Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia. Nat Cell Biol 3, 983-991. PubMed pdf f1000evaluation

Heid, P.J., Raich, W.B., Smith, R., Mohler, W.A., Simokat, K., Gendreau, S.B., Rothman, J.H., and Hardin, J. (2001). The zinc finger protein DIE-1 is required for late events during epithelial cell rearrangement in C. elegans. Dev Biol 236, 165-180. PubMed pdf f1000evaluation

Raich, W.B., Agbunag, C., and Hardin, J. (1999). Rapid epithelial-sheet sealing in the Caenorhabditis elegans embryo requires cadherin-dependent filopodial priming. Curr Biol 9, 1139-1146. PubMed pdf

Williams-Masson, E.M., Heid, P.J., Lavin, C.A., and Hardin, J. (1998). The cellular mechanism of epithelial rearrangement during morphogenesis of the Caenorhabditis elegans dorsal hypodermis. Dev Biol 204, 263-276. PubMed pdf

Raich, W.B., Moran, A.N., Rothman, J.H., and Hardin, J. (1998). Cytokinesis and midzone microtubule organization in Caenorhabditis elegans require the kinesin-like protein ZEN-4. Mol Biol Cell 9, 2037-2049. PubMed pdf

Mohler, W.A., Simske, J.S., Williams-Masson, E.M., Hardin, J.D., and White, J.G. (1998). Dynamics and ultrastructure of developmental cell fusions in the Caenorhabditis elegans hypodermis. Curr Biol 8, 1087-1090. PubMed pdf

George, S.E., Simokat, K., Hardin, J., and Chisholm, A.D. (1998). The VAB-1 Eph receptor tyrosine kinase functions in neural and epithelial morphogenesis in C. elegans. Cell 92, 633-643. PubMed pdf

Costa, M., Raich, W., Agbunag, C., Leung, B., Hardin, J., and Priess, J.R. (1998). A putative catenin-cadherin system mediates morphogenesis of the Caenorhabditis elegans embryo. J Cell Biol 141, 297-308. PubMed pdf f1000evaluation

Williams-Masson, E.M., Malik, A.N., and Hardin, J. (1997). An actin-mediated two-step mechanism is required for ventral enclosure of the C. elegans hypodermis. Development 124, 2889-2901. PubMed pdf

Draper, B.W., Mello, C.C., Bowerman, B., Hardin, J., and Priess, J.R. (1996). MEX-3 is a KH domain protein that regulates blastomere identity in early C. elegans embryos. Cell 87, 205-216. PubMed pdf
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Reviews - C. elegans and adhesion
Walck-Shannon, E. and Hardin, J. (2014). Cell intercalation from top to bottom. Nature Rev. Mol. Cell. Bio 15:34-48. Pubmed pdf

Hardin, J., Lynch, A., Loveless, T., and Pettitt, J. (2013). Cadherins and their partners in the nematode worm Caenorhabditis elegans. In "The Molecular Biology of Cadherins" (ed. F. van Roy). Prog Mol Biol Transl Sci.116:239-62. PubMed pdf

Loveless, T. and Hardin, J.
(2012). Cadherin complexity: recent insights into cadherin superfamily function in C. elegans. Curr. Opin. Cell Biol. 24:695-701. PubMed pdf

Hardin, J.
(2012). An MBoC Favorite: "Cytokinesis and midzone microtubule organization in Caenorhabditis elegans require the kinesin-like protein ZEN-4". Mol. Biol. Cell 23:3025. PubMed pdf

Maiden, S.L. and Hardin, J.
(2011). The secret life of α-catenin: moonlighting during morphogenesis. J. Cell Biol. 195:543–552. PubMed pdf

Schramp, M. and Hardin, J. (2011). Basement remodeling: making way for cellular invaders. Curr. Biol. 21:R585-587. PubMed pdf

Simske, J.S. and Hardin, J. (2011). Claudins in C. elegans. Methods Mol. Biol. 762:147-69. PubMed Draft proof: pdf

Hardin, J.
(2011). Mechanotransduction: Getting morphogenesis down Pat. Curr. Biol. 21:R309-11. PubMed pdf

Lynch, A.M. and J. Hardin.
(2009). The assembly of epithelial junctions in C. elegans. Frontiers Biosci. 14:1414-1432. PubMed pdf

Hardin, J., and King, R. (2008). The long and the short of Wnt signaling in C. elegans. Curr. Opin. Genetics & Devel. 18:362-7. PubMed pdf

Hardin, J. (2008). Preview: To thine own self be true: Self-fusion in single-celled tubes. Dev. Cell 14:465-6. PubMed pdf

Yap, A., Crampton, M.S., and Hardin, J. (2007). Making and breaking contacts: the cellular biology of cadherin regulation. Curr. Opin. Cell Biol. 19:508-514. PubMed pdf

Walston, T. and Hardin, J. (2006). Wnt-dependent spindle polarization in the early C. elegans embryo. Sem. Cell Dev. Biol., 17, 204-213. PubMed pdf

Chisholm, A. and Hardin, J. (2005). Epidermal morphogenesis. In WormBook, ed. The C. elegans Research Community, http://www.wormbook.org PubMed Wormbook (html) pdf

Hardin, J., and Walston, T. (2004). Models of morphogenesis: the mechanisms and mechanics of cell rearrangement. Curr Opin Genet Dev 14, 399-406. PubMed pdf

Hardin, J., and Lockwood, C. (2004). Skin tight: cell adhesion in the epidermis of Caenorhabditis elegans. Curr Opin Cell Biol 16, 486-492. PubMed pdf

Cox, E.A., Tuskey, C., and Hardin, J. (2004). Cell adhesion receptors in C. elegans. J Cell Sci 117, 1867-1870. PubMed pdf

Cox, E.A., and Hardin, J. (2004). Sticky worms: adhesion complexes in C. elegans. J Cell Sci 117, 1885-1897. PubMed pdf

Simske, J.S., and Hardin, J. (2001). Getting into shape: epidermal morphogenesis in Caenorhabditis elegans embryos. Bioessays 23, 12-23. PubMed pdf

Hardin, J. (2000). Development. A degrading way to make an organ. Science 288, 2142-2143. PubMed pdf

Hardin, J., Raich, W.B. and Simske, J.S. (2000) Morphogenesis at single-cell resolution: studying changes in the shape of the embryo in the tradition of Hörstadius. In: "Regulatory Processes in Development" (C.-O. Jacobson, L. Olson, eds) Wenner-Gren International Series, Vol. 76, pp.125-147. Portland Press, London. pdf
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Research articles and reviews - Microscopy
Hardin, J. (2011). Imaging embryonic morphogenesis in C. elegans. In: Joel H. Rothman and Andrew Singson, editors: Methods In Cell Biology, Vol 106, Oxford: Academic Press; 2011, p. 377-412. PubMed pdf

Walston, T. and Hardin, J. (2011). Visualizing cell contacts and cell polarity in Caehorhabditis elegans embryos. In "Imaging in Developmental Biology: A Laboratory Manual" (Sharpe, J., Wong, R.O., eds). Cold Spring Harbor: Cold Spring Harbor Press, pp. 229-244. pdf

Sims, P. A., and Hardin, J. D. (2007). Fluorescence-integrated transmission electron microscopy images: integrating fluorescence microscopy with transmission electron microscopy. Methods Mol Biol 369: 291-308. PubMed pdf

Hardin, J. (2006). Confocal and Multi-Photon Imaging of Living Embryos. In Handbook of Biological Confocal Microscopy, 3e (J. Pawley, ed.). New York: Springer, pp. 746-768. pdf

Sims, P., Albrecht, R., Pawley, J.B., Centonze, V., Deerink, T., and Hardin, J. (2006). When Light Microscope Resolution Is Not Enough: Correlational Light Microscope and Electron Microscope. In Handbook of Biological Confocal Microscopy, 3e (J. Pawley, ed.). New York: Springer, pp. 846-860. pdf

Sims, P., Lockwood, C. and Hardin, J. (2005). Integrating light and TEM information with F-TEM images. Microscopy Today 13(5), 16-18. pdf

Heid, P.J., and Hardin, J. (2000). Cell lineage analysis. Videomicroscopy techniques. Methods Mol Biol 135, 323-330. PubMed pdf

Thomas, C., DeVries, P., Hardin, J., and White, J. (1996). Four-dimensional imaging: computer visualization of 3D movements in living specimens. Science 273, 603-607. PubMed pdf
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Protocols
Walston, T. and Hardin J. (2010). Laser Killing of Blastomeres in Caenorhabditis elegans. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot5543. PubMed pdf

Walston, T. and Hardin, J. (2010). Acquisition of 4D DIC microscopic data to determine cell contacts in Caenorhabditis elegans embryos. Cold Spring Harb. Protoc. doi:10.1101/pdb.prot5541 PubMed pdf

Walston, T. and Hardin J. (2010). An agar mount for observation of Caenorhabditis elegans embryos. Cold Spring Harb. Protoc. doi:10.1101/pdb.prot5540 PubMed pdf

Walston, T. and Hardin J. (2010). Analysis of 4D DIC microscopic data to determine cell contacts in Caenorhabditis elegans embryos. Cold Spring Harb. Protoc. doi:10.1101/pdb.prot5542 PubMed pdf

Research articles - Sea urchins
Hardin, J. and Illingworth, C. (2006). A homologue of snail is expressed transiently in subsets of mesenchyme cells in the sea urchin embryo and is down-regulated in axis-deficient embryos. Dev. Dyn. 235, 3121-31. PubMed pdf

Weng, W., Cheetham , J., Hardin, J., and Venuti, J.M. (2000) A twist in sea urchin gastrulation and mesoderm specification. In: "Regulatory Processes in Development" (C.-O. Jacobson, L. Olson, eds) Wenner-Gren International Series, Vol. 76, pp. 153-158. Portland Press, London. pdf

Kimberly, E.L., and Hardin, J. (1998). Bottle cells are required for the initiation of primary invagination in the sea urchin embryo. Dev Biol 204, 235-250. PubMed pdf

Hardin, J., and Armstrong, N. (1997). Short-range cell-cell signals control ectodermal patterning in the oral region of the sea urchin embryo. Dev Biol 182, 134-149. PubMed pdf

Benink, H., Wray, G., and Hardin, J. (1997). Archenteron precursor cells can organize secondary axial structures in the sea urchin embryo. Development 124, 3461-3470. PubMed pdf

Armstrong, N., Hardin, J., and McClay, D.R. (1993). Cell-cell interactions regulate skeleton formation in the sea urchin embryo. Development 119, 833-840. PubMed pdf

Hardin, J., Coffman, J.A., Black, S.D., and McClay, D.R. (1992). Commitment along the dorsoventral axis of the sea urchin embryo is altered in response to NiCl2. Development 116, 671-685. PubMed pdf
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Reviews - Sea urchins
Hardin, J. (1996). The cellular basis of sea urchin gastrulation. Curr Top Dev Biol 33, 159-262. PubMed pdf

Hardin, J. (1995). Target recognition by mesenchyme cells in the sea urchin embryo. Amer. Zool. 35, 358-371. pdf

Hardin, J. (1994). Local cell-cell interactions and the regulation of gastrulation. Sem. Dev. Biol. 5, 77-84. pdf

Hardin, J. (1994). The sea urchin embryo. In "Embryos: Color Atlas of Development" (J. Bard, ed.), pp. 37-53. London, Wolfe Publishing. pdf

McClay, D.R., Armstrong, N.A., and Hardin, J. (1992). Pattern formation during gastrulation in the sea urchin embryo. Dev Suppl, 33-41. PubMed pdf

McClay, D.R., Morrill, J., and Hardin, J. (1991). Archenteron morphogenesis in the sea urchin. In "Cell-Cell Interactions in Early Development" (J. Gerhart, ed.), New York, Alan R. Liss, pp. 15-29. pdf

McClay, D.R., Alliegro, M.C., and Hardin, J.D. (1989). Cell interactions as epigenetic signals in morphogenesis of the sea urchin embryo. In "The Cellular and Molecular Biology of Pattern Formation" (ed. D. Stocum). Oxford, Oxford University Press, pp. 70-87. pdf

McClay, D.R., Coffman, J.C., and Hardin, J.D. (1989). Epigenetic signals at gastrulation in the sea urchin. U.C.L.A. Symp. Mol. Cell. Biol, New Series, Vol. 25 (eds. E. Davidson, J. Ruderman, and J. Posakony). New York, Alan R. Liss, pp. 251-255. pdf
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Jeff's earlier publications
Hardin, J. (1990). Context-sensitive cell behaviors during gastrulation. Sem. Dev. Biol. 1, 335-345. pdf

Hardin, J., and McClay, D.R. (1990). Target recognition by the archenteron during sea urchin gastrulation. Dev Biol 142, 86-102. PubMed pdf

Hardin, J. (1989). Local shifts in position and polarized motility drive cell rearrangement during sea urchin gastrulation. Dev Biol 136, 430-445. PubMed pdf

Hardin, J., and Keller, R. (1988). The behaviour and function of bottle cells during gastrulation of Xenopus laevis. Development 103, 211-230. PubMed pdf

Hardin, J. (1988). The role of secondary mesenchyme cells during sea urchin gastrulation studied by laser ablation. Development 103, 317-324. PubMed pdf

Keller, R., and Hardin, J. (1987). Cell behaviour during active cell rearrangement: evidence and speculations. J Cell Sci Suppl 8, 369-393. pdf

Hardin, J.D. (1987). Archenteron elongation in the sea urchin embryo is a microtubule-independent process. Dev Biol 121, 253-262. PubMed pdf

Butler, E., Hardin, J., and Benson, S. (1987). The role of lysyl oxidase and collagen crosslinking during sea urchin development. Exp Cell Res 173, 174-182. PubMed pdf

Stephens, L., Hardin, J., Keller, R., and Wilt, F. (1986). The effects of aphidicolin on morphogenesis and differentiation in the sea urchin embryo. Dev Biol 118, 64-69. PubMed pdf

Hardin, J.D., and Cheng, L.Y. (1986). The mechanisms and mechanics of archenteron elongation during sea urchin gastrulation. Dev. Biol. 115 490-501. Abstract pdf
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Teaching Publications & Books
Books
Hardin, J., Bertoni, G., and Kleinsmith, L. (2011). The World of the Cell, 8th ed. San Francisco: Pearson. Amazon

Previous editions:
Becker, W., Kleinsmith, L., Hardin, J. , and Bertoni, G. (2008). The World of the Cell, 7th ed. San Francisco: Benjamin Cummings. Amazon

Becker, W., Kleinsmith, L., and Hardin, J. (2005). The World of the Cell, 6th ed. San Francisco: Benjamin Cummings.

Becker, W., Kleinsmith, L., and Hardin, J. (2003). The World of the Cell, 5th ed. San Francisco: Benjamin Cummings.

Becker, W., Kleinsmith, L., and Hardin, J. (2000). The World of the Cell, 4th ed. San Francisco: Benjamin Cummings.

Articles
Hardin, J. (2008). The missing dimension in developmental biology education. CBE Life Sci Educ. 7:13-6. PubMed pdf

Savage, M.P., Fallon, J.F., and Hardin, J. (2003). Gilbert’s seventh hits all the right notes: A synthesis for everyone. Dev. Dyn. 227, 615–616. Abstract pdf

Savage, M.P., Fallon, J.F., and Hardin, J. (2003). Teaching the essential principles of development. Bioessays 25,301–302. Abstract pdf
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