dlg-1_gfp_cyanA C. elegans embryo undergoing ventral enclosure expressing DLG-1::GFP [Mathias Koeppen].

Ventral epidermal cells migrate ventrally and meet at the ventral midline. Specific cells lead this migration, which is completed when cells make cadherin-dependent contacts along the ventral midline. Click on the links to see example videos…

Ventral enclosure in a wild-type embryo visualized using Nomarski microscopy (Williams-Masson et al., 1997; PubMed). Anterior to the left. Frames were acquired at 2 min. intervals. Original movie footage courtesy of Mathias Koeppen.
Download | Play movie

Ventral enclosure in a wild-type embryo visualized using a dlg-1::gfp translational fusion (Koeppen et al., 2001; PubMed). Frames were acquired at 5 min intervals. Footage courtesy of Mathias Koeppen
Download | Play movie

We are studying:
(1) Protrusive activity at the leading edge of migrating ventral cells; and
(2) How cells create new junctional connections at the ventral midline.